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sp6 promoter  (Azenta)

 
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    Structured Review

    Azenta sp6 promoter
    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and <t>SP6</t> promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.
    Sp6 Promoter, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sp6 promoter/product/Azenta
    Average 86 stars, based on 1 article reviews
    sp6 promoter - by Bioz Stars, 2026-05
    86/100 stars

    Images

    1) Product Images from "Directionality bias in T/A cloning"

    Article Title: Directionality bias in T/A cloning

    Journal: bioRxiv

    doi: 10.64898/2026.02.11.705383

    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and SP6 promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.
    Figure Legend Snippet: A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and SP6 promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.

    Techniques Used: Plasmid Preparation, Cloning, Construct, Generated, Ligation, Selection, Control



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    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and <t>SP6</t> promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.
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    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and <t>SP6</t> promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.
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    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and <t>SP6</t> promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.
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    Image Search Results


    A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and SP6 promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.

    Journal: bioRxiv

    Article Title: Directionality bias in T/A cloning

    doi: 10.64898/2026.02.11.705383

    Figure Lengend Snippet: A) Plasmid map of pGEM-T illustrates the position of T-overhangs used in T/A cloning and flanking T7 and SP6 promoters. B) Percentage of cDNA insertion events in the sense orientation relative to the T7 promoter (yellow) and the SP6 promoter (blue) in constructs generated over the course of four CURE projects. The average across the projects is shown (checkered graph). C) Measured distribution of insert orientation from a cloning attempt of SMEST023171001.1 ( pumilio homolog) cDNA fragment assessed after the ligation and transformant selection steps. D) Bar graphs display orientation distribution of inserts after the ligation step from the cDNA fragment in (C; Gene 1) and two additional genes (SMEST004045004.1, Gene 2; and SMEST012773001.1, Gene 3), as well as their average (checkered bar). Orientation of Control Insert DNA ( luci ) included in the pGEM-T Vector System after the ligation step is also shown. Asterisks show statistical significance according to (* = p < 0.005) the chi-squared test and (*** = p < 0.0005) unpaired Student’s t- test.

    Article Snippet: To tease this apart, a fragment corresponding to a planarian Pumilio homolog (SMEST023171001.1) was amplified from cDNA and ligated into pGEM-T. A portion of the ligation reaction was transformed into Escherichia coli JM109 cells, while another portion of the ligation reaction was used directly as template to sequence across the insertion site between the T7 and SP6 promoter using third generation long read sequencing (PCR-EZ, Azenta).

    Techniques: Plasmid Preparation, Cloning, Construct, Generated, Ligation, Selection, Control